BRED Life Science Technology Inc.
Turn-key Solution for Male Infertility Diagnosis
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Sperm DNA fragment test by flow cytometry (Acridine orange staining kit)
【Clinical significance】
The reagent kit is mainly used to test sperm DNA fragmentation index (DFI).
【Intended use】
The kit is used to evaluate sperm neclear chromatin intactness and maturity.
【Operation process】
1)Test sperm concentration.
2)Adjust sperm concentration 2－3×106 /ml in reagent A.
3)Add with 50ul of reagent A .
4)Add with 100ul of reagent B. React for 30 sec.
5)Add with 300μl of reagent C immediately.
6)Test on flow cytometer (488nm luminescence).