BRED Life Science Technology Inc.
Turn-key Solution for Male Infertility Diagnosis
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Macroscopic observation
Rapid test for sperm superoxide anion (O2-.)
Seminal Reactive Oxygen Species NBT Staining Kit
Applications:
Features:
Test process:
Test results:
The NBT test method is adopted in the form of chromogenic reactions, and the chromogenic product is derived
from the formazan, the reductive substance of tetrazole.Semi-quantitative detection of ROS (O2− •) is based on
the color of formazan.
Diagram of formazan deposit by NBT reaction of Sperm/leukocyte ROS
(a) Negative sperm; (b) Sperm with positive head;
(c) Sperm with positive midpiece; (d) Sperm with positive acrosome;
(e) Weakly positive leukocyte; (f) Strongly positive leukocyte.