Kit to Evaluate Sperm DNA Integrity

Sperm DNA Fragmentation Sperm Chromatin Structure Assay Kit AOT

Flow Cell Methodology

Packing specification:

• 20 tests/kit

Intended use:

• The kit is designed to evaluate the DNA integrity and maturity of human sperm by staining sperm chromatin.

Test principle:

After denatured by acid, fragmented sperm chromatin would turn to single strand. And the normal sperm chromatin retain double strand. Acridine Orange(AO) associated with single-stranded DNA emits red or yellow fluorescence at 488nm luminescence. While AO intercalated in double stranded DNA emits green fluorescene. If the red fluorescence is increasing, it shows a high rate of DNA fragmentation in sperms.

Main compositions:

• Reagent A 1 vial 2.5 ml
• Reagent B 1 vial 4.5 ml
• Reagent C ( lyophilized powder ) 2 vials 13 ml 6.5ml/vial
• Lyophilized powder dissolved solution 1 vial 4 ml

Storage conditions and duration:

• Storage conditions: The kit is kept at 2 ~ 8 ℃ in dark place.
• Period of validity: The kit is kept at 2 ~ 8 ℃ for one year. After dissolved, the reagent C would be kept for two weeks in dark at 2 ~ 8 ℃. Once separately store the dissolved reagent C at -20 ℃ in dark, the use life of reagent C could be more than one year.

Applicable instrument:

• Flow cytometer (488nm luminescence)

Specimen requirements:

• The sample should be collected after a minimum of 48 hours but not longer than seven days of sexual abstinency by masturbation or sexual intercourse using special condoms. The semen sample must be complete in collection.
• Fresh semen samples should be liquefied completely prior to testing. If the samples have delayed liquefaction or high viscosity, a liquefier (sold separately) should be conducted.